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Methods in Molecular Biology Ser.: Neural Crest Cells : Methods and Protocols by Sophie Wiszniak (2019, Hardcover)
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About this product
Product Identifiers
PublisherSpringer New York
ISBN-101493994115
ISBN-139781493994113
eBay Product ID (ePID)23038744252
Product Key Features
Number of PagesX, 230 Pages
LanguageEnglish
Publication NameNeural Crest Cells : Methods and Protocols
Publication Year2019
SubjectNeurology, Life Sciences / Cell Biology, Life Sciences / Developmental Biology
TypeTextbook
Subject AreaScience, Medical
AuthorSophie Wiszniak
SeriesMethods in Molecular Biology Ser.
FormatHardcover
Dimensions
Item Weight24.2 Oz
Item Length10 in
Item Width7 in
Additional Product Features
Series Volume Number1976
Number of Volumes1 vol.
IllustratedYes
Table Of ContentThe neural crest: a remarkable model system for studying development and disease.- Addressing interdisciplinary difficulties in developmental biology / mathematical collaborations: a neural crest example.- Generation of neural crest progenitors from human pluripotent stem cells.- Fully defined and xeno-free induction of hPSCs into neural crest using top-down inhibition of BMP signaling.- Deciphering the neural crest contribution to cephalic development with avian embryos.- In ovo electroporation of plasmid DNA and morpholinos into specific tissues during early embryogenesis.- Ex vivo culture and manipulation of mouse neural crest cells from primary embryonic tissue explants.- Technologies for live imaging of enteric neural crest derived cells.- Mouse embryo culture for the study of neural crest cells.- Laser capture microdissection of murine embryonic neural crest cells.- In vivo and in vitro quantitative analysis of neural crest cell migration.- Proteomics in neural crest cell research.- Neural crest methodologies in zebrafish and medaka.- Fluorescence-activated cell sorting and nanostring profiling of single neural crest cells and pigment cells.- Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity.- Avenues for investigating the neural crest and its derivatives in non-model (unconventional) vertebrates: a craniofacial skeleton perspective.
SynopsisThis detailed book provides a wide range of techniques, from those that have been used extensively since the very first investigations into neural crest cells to those that are currently cutting-edge, in order to explore the development of neural crest cells in human, mice, rat, chick, quail, medaka, and shark. With a bit of imagination and adjustment, many of these methodologies can be adaptable to any species desirable for study. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neural Crest Cells: Methods and Protocols serves as an ideal reference guide for aspiring and experienced developmental biologists alike.
LC Classification NumberQH491-492
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