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Methods in Molecular Biology Ser.: Chromosomal Mutagenesis by Kevin J. Kayser (2007, Hardcover)
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About this product
Product Identifiers
PublisherHumana Press
ISBN-10158829899X
ISBN-139781588298997
eBay Product ID (ePID)16038376881
Product Key Features
Number of PagesXvi, 236 Pages
Publication NameChromosomal Mutagenesis
LanguageEnglish
Publication Year2007
SubjectLife Sciences / Cell Biology, Life Sciences / Biochemistry, Life Sciences / Genetics & Genomics, Genetics
TypeTextbook
AuthorKevin J. Kayser
Subject AreaScience, Medical
SeriesMethods in Molecular Biology Ser.
FormatHardcover
Dimensions
Item Height0.2 in
Item Weight19.3 Oz
Item Length9.3 in
Item Width6.1 in
Additional Product Features
Intended AudienceScholarly & Professional
ReviewsFrom the reviews:"I looked at Chromosomal Mutagenesis from the perspective of a geneticist … . it serves as a potentially useful text. … well written and protocols appear very detailed with good explanation that goes well beyond what one can glean from most published papers. … On the whole, for anyone either thinking of moving into a field where chromosomal mutagenesis is not so straightforward, or looking for ideas by learning how others have solved related problems, this book could serve as a very useful resource." (Alistair Goldman, Microbiology Today, May, 2008), From the reviews: "I looked at Chromosomal Mutagenesis from the perspective of a geneticist ... . it serves as a potentially useful text. ... well written and protocols appear very detailed with good explanation that goes well beyond what one can glean from most published papers. ... On the whole, for anyone either thinking of moving into a field where chromosomal mutagenesis is not so straightforward, or looking for ideas by learning how others have solved related problems, this book could serve as a very useful resource." (Alistair Goldman, Microbiology Today, May, 2008)
Dewey Edition22
Series Volume Number435
Number of Volumes1 vol.
IllustratedYes
Dewey Decimal572.87
Table Of ContentBiallelic Gene Knockouts in Chinese Hamster Ovary Cells.- Highly Proficient Gene Targeting by Homologous Recombination in the Human Pre-B Cell Line Nalm-6.- Robust Cell Line Development Using Meganucleases.- Design and Testing of Zinc Finger Nucleases for Use in Mammalian Cells.- Gene Targeting in Drosophila and Caenorhabditis elegans With Zinc-Finger Nucleases.- Orpheus Recombination.- Transposon-Mediated Mutagenesis in Somatic Cells.- Insertional Mutagenesis of the Mouse Germline With Sleeping Beauty Transposition.- Conditional Gene Trapping Using the FLEx System.- Steps Toward Targeted Insertional Mutagenesis With Class II Transposable Elements.- Targeting Integration of the Saccharomyces Ty5 Retrotransposon.- Site-Specific Chromosomal Integration Mediated by ?C31 Integrase.- Triplex-Mediated Gene Modification.- Allelic Exchange of Unmarked Mutations in Mycobacterium Tuberculosis.- Mycobacterial Recombineering.- Chromosomal Engineering of Clostridium Perfringens Using Group II Introns.
SynopsisGreat disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation. In this book, a team of experts provide a variety of chromosomal manipulation techniques and attempt to expand the genetic toolbox beyond model organisms. Following the format of the highly successful Methods in Molecular Biology(TM) format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, this book covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types., Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation. In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms. Following the format of the highly successful Methods in Molecular Biology? format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Chromosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types., Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation. In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms. Following the format of the highly successful Methods in Molecular Biology(tm) format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Chromosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types., Great disparities exist between organisms with regard to the relative ease of chromosomal mutagenesis and manipulation. In Chromosomal Mutagenesis, a team of experts provide a variety of chromosomal manipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms. Following the format of the highly successful Methods in Molecular Biology(TM) format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Chromosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types.
LC Classification NumberQD415-436
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