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YAC PROTOCOLS (METHODS IN MOLECULAR BIOLOGY) By David Markie Excellent Condition
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eBay item number:186390742115
Item specifics
- Condition
- Very Good
- Seller Notes
- ISBN-10
- 0896033139
- Publication Name
- Humana Press
- Type
- Paperback
- ISBN
- 9780896033139
- Book Title
- Yac Protocols
- Book Series
- Methods in Molecular Biology Ser.
- Publisher
- Humana Press
- Item Length
- 9 in
- Publication Year
- 1995
- Format
- Other
- Language
- English
- Illustrator
- Yes
- Item Height
- 1.1 in
- Genre
- Science, Medical
- Topic
- Life Sciences / Molecular Biology, Life Sciences / Genetics & Genomics, Genetics
- Item Weight
- 22.6 Oz
- Item Width
- 6 in
- Number of Pages
- 384 Pages
About this product
Product Information
Yeast Artificial chromosomes (YACs) have emerged as a powerful technology for cloning very large pieces of DNA. This collection of protocols makes YACs accessible to investigators who are already using them, or who lack a background in yeast. The text follows the standard format of all of the MiMB books by having step-by-step protocols followed by a notes section. The techniques have been laboratory tested to ensure success.
Product Identifiers
Publisher
Humana Press
ISBN-10
0896033139
ISBN-13
9780896033139
eBay Product ID (ePID)
509150
Product Key Features
Book Title
Yac Protocols
Number of Pages
384 Pages
Language
English
Publication Year
1995
Topic
Life Sciences / Molecular Biology, Life Sciences / Genetics & Genomics, Genetics
Illustrator
Yes
Genre
Science, Medical
Book Series
Methods in Molecular Biology Ser.
Format
Other
Dimensions
Item Height
1.1 in
Item Weight
22.6 Oz
Item Length
9 in
Item Width
6 in
Additional Product Features
Dewey Edition
20
Lccn
95-020892
Series Volume Number
Vol. 54
Target Audience
Trade
Dewey Decimal
575.1/0724
Lc Classification Number
Qh442.2.Y33 1996
Table of Content
Generation of Large Insert YAC Libraries. YAC Library Storage and Transport. YAC Library Screening I: Preparation of Hybridization Filters and PCR Pools. YAC Library Screening II: Hybridization and PCR-Based Screening Protocols. Cloning of Human Telomeres in Saccharomyces cerevisiae. Purification of YAC-Containing Total Yeast DNA. Restriction Analysis of YACs. RARE-Cleavage Analysis of YACs. YAC Localization by Fluorescence In Situ Hybridization. Alu-PCR Fingerprinting of YACs. Detection of Chimerism in YAC Clones. Amplification with Arbitrary Primers. End Rescue from YACs Using the Vectorette. Isolation of YAC Ends by Plasmid Rescue. End-Rescue of YAC Clone Inserts by Inverse PCR. Covering YAC-Cloned DNA with Phages and Cosmids. Fragmentation and Integrative Modification of YACs. Targeting Mutations to YACs by Homologous Recombination. Reconstruction of Large Genomic Segments of DNA by Meiotic Recombination Between YACs. Genomic Reconstruction by Mitotic Recombination of YACs. Amplification of the Copy Number of YACs. Transfer of YAC Clones to New Yeast Hosts. Use of ACEDB as a Database for YAC Library Data Management. YAC Transfer into Mammalian Cells by Cell Fusion. YAC Transfer by Microinjection. Transfection of Mammalian Cells via Lipofection. The Isolation of cDNAs by Hybridization of YACs to cDNA Libraries. cDNA Selection with YACs. Markers Selection and Media in YAC Cloning. Index.
Copyright Date
1996
Item description from the seller
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eBay item number:186390742115
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